细胞分析

从生物样品中快速、高效且灵敏地筛选和捕获细胞对深入了解复杂的结构、生理和分子机制至关重要。采用Bio-Rad仪器、试剂盒、试剂和软件，可以有效地筛选、捕获和标记细胞，或者回收细胞进行培养或进行NGS、ddPCR、qPCR、流式分析或蛋白印迹等下游分析，同时保留细胞的原始生物学特性。

Genesis系统

Genesis采用温和的微流控技术，基于孔径筛选原理可轻松捕获、回收粒径在8-30μm范围的细胞。本系统配有直观的触摸屏界面，采用集成式分液技术和玻片温控技术。

The Genesis System with Celselect Slides™ enable the capture of cells from liquid biopsies and other sample types.

The Celselect Slides utilizes 140,800 individual microchambers with a pore size that captures cells in the range of 8-30 µm (figure 1):

Illustration of the microfluidic chambers on the Celselect Slides

llustration of the microfluidic chambers on the Celselect Slides with a blood sample example showing capture of CTCs while smaller blood components (<8 µm) flow through.

In Figure 2, red blood cells and smaller white blood cells flow through the pores while cells larger than 8 µm are captured. Circulating tumor cells (CTCs) and CTC clusters for the majority of cancers types are captured by the Celselect Slides.

Genesis System Figure 2

Figure 2

Blood cell and CTC sizes relative to the Celselect Slides pore size.

After capture, the enriched cells can be recovered for downstream analysis such as single-cell genomics, digital PCR, or culture. Alternatively, cells can be immunolabeled on-slide for immunofluorescent applications such as enumeration.

For applications which require protocol optimization or creation of new protocols for custom reagents or antibody labeling, the Genesis Protocol Builder Software Tool enables adjustment of parameters such as reagent dispensing, pump pressures, and incubation settings. Building custom protocols is performed on your local computer.

Features and Benefits

Size-based cell capture from liquid biopsies and other sample types

Recover viable cells for downstream analysis or on-slide immunolabeling for enumeration

Genesis Protocol Builder Software Tool enables optimization for different samples types or antibody stains

Celselect Slides Enumeration/Immunolabeling Workflow

Sample is prepared with fixing reagent and loaded on to the instrument. 8-30 µm sized cells are captured and immunolabeled automatically in the Celselect Slides microchambers while smaller components flow through the slide to waste. The Celselect Slides is imaged on an automated microscope and files can be imported for reporting.