ddSEQ™ Single-Cell 3' RNA-Seq Kit

ddSEQ Single-Cell 3' RNA-Seq Kit & Omnition Analysis Software

The ddSEQ 3’ RNA-Seq Library Prep Kit allows researchers to profile gene expression with single-cell precision.

The ddSEQ Single-Cell 3’ RNA-Seq Kit and Omnition Analysis Software, together, enables streamlined whole transcriptome analysis (WTA) at the single-cell level. Leveraging Bio-Rad’s ddSEQ droplet technology, this assay is able to capture and profile hundreds to thousands of single cells with high sensitivity and recovery.

Features & Benefits

Simple & fast encapsulation — Cells and nuclei are encapsulated in under 5 minutes using ddSEQ droplet partitioning technology

Essential performance — Delivers the required sensitivity and utilization with low multiplet rates, ensuring confidence in successful 3' RNA-seq applications

Adjustable throughput — Flexible processing of 500 to 5,000 cell output per sample, accommodating a range of scale

Streamlined assay workflow — Reduced workflow time with convenient stopping points, enabling completion within a day or at your convenience

Advanced informatics — Efficient and flexible informatics tool, featuring batch pipeline analysis of samples and a comprehensive single-cell metrics report, powered by Omnition Analysis Software

Applications & Uses

Uncover cellular heterogeneity in biological samples and tissues

Identify rare or novel cell types associated with pathogenesis or as biomarkers

Understand developmental pathways and trajectories

Included Components

ddSEQ Single-Cell 3' RNA-Seq Kit (8 reactions)

ddSEQ Single-Cell 3' RNA-Seq cDNA Module [includes: Droplet Generation Module (4°C) and RT Module (–20°C)]

ddSEQ Single-Cell 3' RNA-Seq Library Module

ddSEQ M Cartridges (2 cartridges)

Omnition Analysis Software version 1.1+