ProteoMiner™ Protein Enrichment Large-Capacity Kit 1633007

ProteoMiner™ Protein Enrichment Large-Capacity Kit #1633007

10 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 10 spin columns, wash buffer, elution reagents, collection tubes

Use ProteoMiner Protein Enrichment Large-Capacity Kits to simultaneously concentrate low-abundance proteins and lower the concentration of high-abundance proteins in ≥50 mg of sample. The protein complexity of the sample is maintained.

The enrichment protocol uses a large, highly diverse library of combinatorial peptide ligands bound to beads. After high-abundance proteins have saturated all their high-affinity ligands, the unbound excess protein is washed away. Most medium- and low-abundance proteins will not saturate their ligands, resulting in no excess to be washed away.

Knowledge of the identity and characteristics of the proteins within a sample is not required

Compatible with a wide range of sample types

Narrows the range of protein concentrations within a sample

Can be used for differential expression analysis

Compatible with most downstream applications including 1-D and 2-D electrophoresis and mass spectrometry

For fractionation of the protein sample after binding to the beads, the ProteoMiner Protein Enrichment Small-Capacity Kit can be used with the ProteoMiner Sequential Elution Reagents (1633003). The elution kit contains four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics. The ProteoMiner Protein Enrichment Large-Capacity Kit and Sequential Elution Reagents can be purchased together in one kit, the ProteoMiner Sequential Elution Large-Capacity Kit (1633011). Sequential elution has been optimized for samples for SELDI. Elution reagents are not compatible with direct use of fractions in 2-D gel electrophoresis.

Kit Contents

Spin columns, 10; 500 µl bead slurry (20% beads, 20% v/v EtOH), 100 µl settled bead volume

Wash Buffer, 150 mM NaCl, 10 mM NaH2PO4, pH 7.4, 50 ml

Elution Reagent, lyophilized 8 M urea, 2% CHAPS, 5 vials

Rehydration Reagent, 5% acetic acid, 5 ml

Capless 2 ml centrifuge tubes, 20

Capped 2 ml centrifuge tubes, 10

Instruction Manual

Quick Start Guide