活细胞成像试剂

用于活细胞成像的细胞运输和标记试剂。

实时的活细胞成像可使科学家能够对无法使用传统的终点技术（例如固定成像和PCR仅能捕获一个静态的时刻）观察的动态细胞事件进行监测。我们产品的活细胞成像试剂组合包括用于细胞示踪的新型荧光细胞标记技术、荧光慢病毒生物传感器、传统荧光染料和用于实时分析细胞事件的探针。这些试剂适于通过CellASIC® ONIX2微流体活细胞成像系统等实时孵育和成像系统捕获动态细胞事件。

PKH和CellVue®荧光细胞连接子标记物

LentiBrite™荧光慢病毒生物传感器

BioTracker活细胞荧光染料和染色剂

BrightCell™光稳定培养基

LuminiCell Tracker™荧光纳米粒子

Absorbance Maximum: 510nm

Emisson Maximum: 670nm

Suggested Filters:

Excitation: 458/488/543nm

Emisson: 670-800nm

Long-term noninvasive cell tracking by fluorescent probes and quantum dots is of great importance to life science and biomedical engineering. Current methods used to fluorescently tag cells have been limited by short signal duration, high background auto-fluorescence or lengthy molecular cloning manipulations using GFP. LuminiCell Trackers<TMSYMBOL></TMSYMBOL> are biocompatible organic fluorescent nanoparticles based on Aggregation Induced Emission technology, termed AIEDots. Aggregation induced emission (AIE) molecules emit fluorescence in an opposite manner than other common fluorophores (Quantum Dots, GFP). Propeller-shaped AIE fluorogens are non-emissive in solutions but become highly fluorescent upon aggregate formation. Due to these differences, LuminiCell Trackers<TMSYMBOL></TMSYMBOL> have very high fluorescence intensities with minimal signal quenching allowing live cell fluorescent tagging for up to 10 days in vitro and 21 days in vivo. These properties make them optimal candidates for long interval live cell bioimaging experiments.

The Luminicell Tracker<TMSYMBOL></TMSYMBOL>-670 Vascular Labeling Kit contain red fluorescent AIEDots nanoparticles without TAT sequences. These nanoparticles can be used to fluorescently tag vasculature in living tissues and animals for studies of inflammation and vascular leakage.

Features and Benefits

● Brighter: 10X brighter than other cell labeling technologies

● Photostable: 3X longer fluorescence without signal quenching

● Biocompatible: Non-toxic organic nanoparticles intended for biological applications

● Rapid Protocol: Easy-to-use protocol labels cells within 4 hours

质量

Absorbance: 510+/-5nm

Concentration: 180-220nM

Fluorescence: 665+/-10nm

Brightness at 670nm: ≥1.70X10^7 M-1cm-1

外形

500nM in 0.5ml PBS, pH 7.4

储存及稳定性

Store at -2-8°C upon receipt. Thaw at room temperature or in a water bath. Do not Freeze.

Note: Some particulates may form as a result of nanoparticle aggregation during shipping. To get particulates back in solution, sonicate the vial containing LuminiCell Tracker three times for 1 min each before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.