羟基磷灰石树脂,DNA级Bio-Gel HTP,100g价格_品牌:伯乐biorad 1588000 羟基磷灰石10G装

美国伯乐BIO-RAD CHT陶瓷羟基磷灰石

CHT 陶瓷羟基磷灰石（原先称为Macro–Prep 陶瓷羟基磷灰石）是一种球形、 巨孔的羟基磷灰石。它避免了晶体材料的局限性，可用于工业级层析柱。这种陶瓷材 料的分离方案与晶体羟基磷灰石的基本一致。CHT 既保留了晶体羟基磷灰石独特的 分离特性，同时又可在高流速大型层析柱中反复使用，达数百循环。

I 型和II 型CHT 陶瓷羟基磷灰石具有晶体羟基磷灰石类似的洗脱特性，但同时也 有3个重要差异。I型的蛋白载量高，且对酸性蛋白载量更高。II型的蛋白载量较低， 对早期洗脱蛋白以及核酸的分辨率较佳。由于II型对白蛋白的亲和力很低，它通常比I 型更适于纯化多种类的免疫球蛋白。3 种颗粒大小20, 40 和80μm 能广泛地用于分析 规模到制备规模的应用。

【技术指标】

Chemical Compatibility Type I Type II

1 M NaOH >24 hr >24 hr

8 M urea >24 hr >24 hr

8 M guanidine-HCl >24 hr >24 hr

Ethanol or methanol >24 hr >24 hr

100% acetonitrile >24 hr >24 hr

A small amount of an appropriate counterion such as 0.1–10 mM NaPO4– should be added to all unbuffered solutions.

Properties

Functional groups Ca2+, (PO4)3–

Particle sizes available 20, 40, and 80 µm (nominal)

Recommended operating flow rates 10–1,500 cm/hr

pH stability 5.5–14

Chemical compatibility (>24 hr) 1 M NaOH, 8 M urea, 8 M guanidine-HCl, ethanol, methanol, 100% acetonitrile

Regeneration 0.4–1.0 M phosphate buffer

Sanitization 1–2 M NaOH

Autoclavability (121°C, 20 min) Yes

Packing density (g/ml packed bed) 0.56 g/ml

Capacity Type I Type II

Dynamic binding >25 mg lysozyme/g >12.5 mg lysozyme/g

Typical IgG binding at 500 cm/hr 10–50 mg/ml 5–25 mg/ml

Nominal pore diameter 600–800 Å 800–1,000 Å

Maximum operating pressure 60 bar (900 psi) 100 bar (1,500 psi)

【订货信息】

目录 # 描述

CHT Ceramic Hydroxyapatite, Type I

157-0020 CHT Ceramic Hydroxyapatite, Type I, 20 µm particle size, 100 g

157-0021 CHT Ceramic Hydroxyapatite, Type I, 20 µm particle size, 1 kg

157-0025 CHT Ceramic Hydroxyapatite, Type I, 20 µm particle size, 5 kg

157-0040 CHT Ceramic Hydroxyapatite, Type I, 40 µm particle size, 100 g

157-0041 CHT Ceramic Hydroxyapatite, Type I, 40 µm particle size, 1 kg

157-0045 CHT Ceramic Hydroxyapatite, Type I, 40 µm particle size, 5 kg

157-0080 CHT Ceramic Hydroxyapatite, Type I, 80 µm particle size, 100 g

157-0081 CHT Ceramic Hydroxyapatite, Type I, 80 µm particle size, 1 kg

157-0085 CHT Ceramic Hydroxyapatite, Type I, 80 µm particle size, 5 kg

158-2000 CHT Ceramic Hydroxyapatite, Type I, 20 µm particle size, 10 g

158-4000 CHT Ceramic Hydroxyapatite, Type I, 40 µm particle size, 10 g

158-8000 CHT Ceramic Hydroxyapatite, Type I, 80 µm particle size, 10 g

CHT Ceramic Hydroxyapatite, Type II

157-2000 CHT Ceramic Hydroxyapatite, Type II, 20 µm particle size, 100 g

157-2100 CHT Ceramic Hydroxyapatite, Type II, 20 µm particle size, 1 kg

157-2500 CHT Ceramic Hydroxyapatite, Type II, 20 µm particle size, 5 kg

157-4000 CHT Ceramic Hydroxyapatite, Type II, 40 µm particle size, 100 g

157-4100 CHT Ceramic Hydroxyapatite, Type II, 40 µm particle size, 1 kg

157-4500 CHT Ceramic Hydroxyapatite, Type II, 40 µm particle size, 5 kg

157-8000 CHT Ceramic Hydroxyapatite, Type II, 80 µm particle size, 100 g

157-8100 CHT Ceramic Hydroxyapatite, Type II, 80 µm particle size, 1 kg

157-8500 CHT Ceramic Hydroxyapatite, Type II, 80 µm particle size, 5 kg

158-2200 CHT Ceramic Hydroxyapatite, Type II, 20 µm particle size, 10 g

158-4200 CHT Ceramic Hydroxyapatite, Type II, 40 µm particle size, 10 g

158-8200 CHT Ceramic Hydroxyapatite, Type II, 80 µm particle size, 10 g

Note: Larger volumes and special packaging for industrial applications are available on request.